http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114958991-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_811614b5f22cf41587a0cc6494b4b806 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e45dc66f621b884f7ba968a9d0157276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25f2ffa4a46914ca205c4b4d44f32815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ff5ab4ee4b5cebb6cee105f93dab8b5 |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114958991-A |
| titleOfInvention | An ultra-high-throughput multiplex PCR amplicon capture method |
| abstract | The present invention relates to the field of second-generation sequencing, in particular to an ultra-high-throughput multiplex PCR amplicon capture method for second-generation sequencing. The NGS capture method includes the following steps: 1) using a digestible composite modified primer to be detected 2) Use the second enzyme mixture containing primer digestion enzyme, error correction enzyme and end repair enzyme to carry out enzyme digestion reaction on the PCR product, including the digestion enzyme used to remove the amplification product For the redundant primer part, use a mismatch correction enzyme to remove non-specific amplification products; 3) Use a full-length linker containing Index to perform a ligation reaction to directly obtain a library for sequencing or obtain a library for sequencing after amplification. The method of the invention significantly improves the amplification efficiency and specificity, and has the advantages of simple steps, low cost and short time, and has wide application value in the fields of gene detection and scientific research such as genetic disease detection, tumor accompanying diagnosis and the like. |
| priorityDate | 2022-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.