http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114958985-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_23a5342bae16d29b8e5e9877dc168e34 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2022-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_936d2b59dd410cad1253a265f3d99a85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c9e2ad22c013b4e2d4689f3bc0fcd06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67cb63936fbdda7dc43d6648d14ef106 |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114958985-A |
| titleOfInvention | A multiplex PCR method, primer-probe combination and kit for detecting PCM1-JAK2 fusion gene |
| abstract | The invention relates to the technical field of biomedicine, and provides a multiplex PCR method for detecting a PCM1-JAK2 fusion gene, a primer-probe combination and a kit. The primer-probe combination includes a primer-probe combination A1 and a primer-probe combination A2: primer-probe combination A1 and primer-probe combination A2: Needle set A1 includes upstream primers PCM1‑E23‑F, PCM1‑E26‑F, PCM1‑E28‑F, PCM1‑E30‑F, PCM1‑E36‑F, downstream primers JAK2‑E9‑R, JAK2‑E12‑R and Probes JAK2‑E9‑Probe, JAK2‑E12‑Probe, primer probe set A2 includes upstream primers PCM1‑E23‑F, PCM1‑E26‑F, PCM1‑E28‑F, PCM1‑E30‑F, PCM1‑E36‑F F. Downstream primer JAK2‑E11‑R and probe JAK2‑E11‑Probe, the kit includes PCR reaction solution of detection system and lymphocyte separation solution. By dividing the primer probe into two combinations, it can cover various fusions to the maximum extent The primer-probe combination designed by the present invention can simultaneously detect known fusion methods and possible unknown fusion methods, and can detect various fusion methods at the same time, reducing the detection cost; The multiplex PCR technology of the probes ensures the specificity of detection and amplification. |
| priorityDate | 2022-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.