| abstract |
The present application discloses an evaluation model for idiosyncratic liver injury, the evaluation model includes AIM2 inflammasome and components that promote the activation of AIM2 inflammasome, wherein, promoting bakuchiol as an immune enhancer and inducing mitochondrial DNA release, The component that leads to the activation of the AIM2 inflammasome is bakuchiol. The present application also provides a method for constructing the evaluation model, which includes: preparation of a psoralen monomer sample solution; LPS pretreatment of mouse bone marrow-derived primary macrophages, and adding LPS to induce 4 hours; The lipid monomer solution was added to mouse bone marrow-derived primary macrophages for stimulation; Western blot was performed to detect AIM2 inflammasome activation-related proteins, ELISA to detect the release of inflammatory factors IL-1β and TNF-α, and statistical analysis was performed. |