abstract |
The invention relates to the in vitro preparation, activity detection and application of an antifungal drug action target, and belongs to the field of drug target material and its preparation and application. The drug target in the present invention is the key enzyme FKS (β-1,3-glucan synthase) that inhibits fungal cell wall synthesis. The active drug target protein was prepared in vitro through the eukaryotic expression system, and an effective enzyme activity analysis system was established by using its feature of converting the substrate into UDP (uridine diphosphate), which can be used to detect inhibitory Enzyme activity of drugs or compounds, the system has the feasibility of drug screening; in addition, the application of FKS enzyme activity detection method in the identification of fungal drug-resistant mutants is also carried out. |