| abstract |
The invention discloses a method for preparing a transfer factor of rabbit round vesicles. 1) mixing water for injection with minced rabbit round vesicles, and homogenizing by a colloid mill to obtain a homogenate; 2) after the homogenate is disrupted by cells, Centrifuge, and tangential flow filtration of the harvested supernatant to collect the permeate; 3) The permeate is subjected to tangential flow filtration step by step with filter membranes with different cut-off pore sizes and molecular weights to obtain rabbit circles with the desired molecular weight range. 4) Mixing at least one crude product with a molecular weight range in step 3), that is, the first preparation of rabbit vesicle transfer factor; 5) After removing the virus from the first preparation of rabbit vesicle transfer factor, Adjust pH and osmotic pressure, filter and sterilize, and obtain rabbit round capsule transfer factor. The invention adopts the step-by-step tangential flow filtration, and establishes an accurate and effective technology for separating and concentrating the transfer factor of the rabbit round vesicles and purifying the small molecule. Rabbit oval transfer factor with different molecular weight ranges. |