http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114942325-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb117a460191c60397dfeb1c84b12b2b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1081 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 |
| filingDate | 2022-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5394c93f0ee05f03dbcbf8ba9b66a4e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d5d048a12123b069eb49360de992e8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cf5a346492de2d21d1ff3af3a5dfccc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ae54c2f4eaa6c53d380d845b0b8d7a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_651100788d164fdb16e0596c2cb83348 |
| publicationDate | 2022-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114942325-A |
| titleOfInvention | Method for quantitatively detecting Japanese encephalitis virus antigen content, monoclonal antibody prepared for establishing the method and application |
| abstract | The present application relates to the technical field of biological detection, in particular to a method for quantitatively detecting the antigen content of Japanese encephalitis virus, a monoclonal antibody prepared for establishing the method and its application. The method of the present application includes the following steps: coating JE monoclonal antibody on an enzyme labeling plate, adding JE positive reference substance, negative control and diluted sample to be tested by gradient dilution to each well after the coating is completed, washing after incubation ELISA plate, add enzyme-labeled JE monoclonal antibody, wash the ELISA plate after re-incubating, add substrate chromogenic solution to develop color, stop the microplate reader to read the OD value, and draw the regression with the OD value of the JE positive reference substance Equation, the content of Japanese encephalitis virus antigen in the vaccine was quantitatively obtained by regression calculation. Therefore, the method of the present application can effectively and accurately predict the antigen content of the finished vaccine, provide a reliable quantitative numerical basis for the production of the JE vaccine, and can be better applied to the quality control of the JE vaccine production. |
| priorityDate | 2022-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 271.