| abstract |
The invention discloses the construction of a 4-1BB reporter gene 293T stable transfection cell line. and monoclonal selection, and after functional evaluation, a suitable 4-1BB reporter gene stably transfected cell line was obtained. And discloses a 4-1BB agonist drug biological activity assay method and a neutralizing antibody assay method using the 4-1BB reporter gene 293T stable transfection cell line, which can fully characterize the 4-1BB activation mechanism and effect or the detection steps are simple and time-consuming. Low cost, low cost of reagent consumables, and high method stability. |