http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114940698-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_71d340034c21d634d96592f92a4d5707 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6188908b5a711cc6be0aad9fd38a9214 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe8b5c099df8a10eb5c560b4d9f367b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfe113e65eede19be7cec94045107802 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2277ed8c94ef3b253f88dfc14c7051a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff324f02aba1caf5659adc152108139c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48c4c590e053f47dc00b3b4c21f4a4cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bde157793a1be6558ca497feb27fae47 |
| publicationDate | 2022-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114940698-A |
| titleOfInvention | A kind of extraction method of acidic polypeptide in spleen aminopeptide stock solution |
| abstract | The invention relates to a method for extracting acidic polypeptides from spleen aminopeptide stock solution. The extraction method includes the following steps: (1) sample pretreatment: take a frozen sample of spleen aminopeptide stock solution, thaw, centrifuge to discard the precipitation, and obtain a spleen aminopeptide pretreatment solution; (2) chromatographic separation: use a buffer solution A equilibrates the chromatography column, the spleen aminopeptide pretreatment solution in step (1), continues to wash with buffer A, and collects the flow-through; The eluate was collected. The invention adopts anion exchange chromatography to efficiently separate the spleen aminopeptide acidic polypeptide, the separation process is simple, and the separation time is short, thereby obtaining the spleen aminopeptide acidic polypeptide with high biological activity, and the isoelectric point of the acidic polypeptide is detected by full-column imaging capillary electrophoresis: 3.38. After biological activity evaluation, the eluate obtained by the extraction method provided by the present invention has higher specific activity than the spleen aminopeptide stock solution, indicating that it has higher biological activity. |
| priorityDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.