| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_acdc9a41903b38b43b216a9b473d332b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2570-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-179
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-205 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54366
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6818 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6813 |
| filingDate |
2020-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fed4b9c0e2b9e0774bbd2f86a145a1f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74596653b2ee3ef910bfdbafcd72bd82 |
| publicationDate |
2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114929887-A |
| titleOfInvention |
Methods for sequencing and reconstruction of single peptides |
| abstract |
Methods for sequencing single peptides. The method includes providing an enriched sample comprising a population of polypeptides; dividing the enriched sample into two or more sub-samples; contacting each of the at least two sub-samples with a different modifier, wherein the modifier includes a lysing agent, such as an external peptidase to generate polypeptide fragments having a combination of cleavage modes; and parallel sequencing of the polypeptide fragments to determine the amino acid sequence of the polypeptide fragments. Fragments can be aligned to reconstruct polypeptide sequences. Also provided herein are kits comprising multiple enrichment molecules, such as antibodies, aptamers or enzymes, and sample preparation devices comprising barcodes and capture probes. |
| priorityDate |
2019-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |