Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a4c1454d8ca3bba80b9acc438ee942f |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70596 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-705 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57492 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate |
2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e22be32518e11311e09c33e06cc17e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_163e23d924991bba2f9643df13f06bbf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_250f86810f3ada9de6a419dd6e2ab3dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48cce3f33164e54c4420795773065221 |
publicationDate |
2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-114924083-A |
titleOfInvention |
A nucleic acid aptamer-based detection method and application of fluorescence polarization of extracellular vesicles |
abstract |
The present invention relates to the technical field of C12N15/115, in particular to a nucleic acid aptamer-based extracellular vesicle fluorescence polarization detection method and application thereof. It includes the following steps: S1. Immobilize EVs by reacting antibodies with EV surface biomarker proteins or EV surface cancer markers; then quickly wash to remove free EVs, proteins, membrane debris and lipids; S2. Add and EV markers respectively The nucleic acid aptamer is fluorescently labeled; S3. Fluorescence polarization detection is performed on the product of step S2 to achieve qualitative and quantitative analysis of EVs secreted by cancer cells. The invention can specifically detect extracellular vesicles secreted by cancer cells in blood, the detection process is not interfered by free tumor marker proteins, tumor cell membrane fragments or tumor extracellular vesicle membrane fragments in blood, and the detection results are accurate and effective. |
priorityDate |
2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |