| abstract |
The invention relates to the technical field of biomedicine, in particular to a detection method and application of cobra toxin and viper toxin. (1) Antibodies obtained from immunization with a mixture of SVSP chimera and Disintegrin chimera and two antigens of 3FT chimera can effectively identify cobra toxin and viper toxin; (2) Using multicinctus 3FT chimera, Atra 3FT The antibodies obtained by immunizing the two antigens of the chimera can effectively identify cobra toxin and bungee venom; (3) The use of cation exchange chromatography to enrich the snake venom in the sample improves the detection of snake venom in blood and tissue fluid. Sensitivity; (4) Cobra and silver ring snake venom standards were established for the first time. Compared with the prior art, it is possible to search for differential proteins from viper venom and cobra venom, predict their linear epitopes, remove the same and highly similar linear epitopes, and recombine them in tandem, which has the ability to identify Viper venoms. The role of snake venom and cobra venom. |