| abstract |
The invention discloses a method for detecting the fingerprint of A. chinensis, which comprises the following steps: step 1, preparation of a test solution of C. chinensis; step 2, preparation of a mixed reference solution; step 3, investigation of a linear relationship; 4. Precisely draw the test solution and inject it into the liquid chromatograph, and record the chromatogram; Step 5, export the fingerprint of the Digum chinensis test solution obtained in the step 4, and import it into the Chinese Medicine Chromatography Fingerprint Similarity Evaluation System 2012A , to generate the characteristic fingerprint of D. chinensis; and by comparing the retention time of each peak and the chromatographic peak of the mixed reference substance, the characteristic fingerprint of D. chinensis was assigned. The detection method provided by the present invention can comprehensively and objectively characterize the quality of the grass. And it has the advantages of good stability, good reproducibility, high precision and accuracy. |