http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114921533-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a9c98c6fc482c4bfca164bcff2635c90 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2022-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14d08cf2ee82ba3c10de33231d60fa33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3050797dc33ebe65c28e04bb48d3b1ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bbc6bc9d7497a0551d56b3aada99112 |
| publicationDate | 2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114921533-A |
| titleOfInvention | Methods and adapters for characterizing target polynucleotides |
| abstract | The present invention provides methods and adapters for characterizing polynucleotides of interest. The present invention provides a method of characterizing a target polynucleotide, comprising: (a) moving the target polynucleotide through a nanopore, wherein the sequencing terminus of the target polynucleotide comprises a modified portion that enables Nanopore plugging; (b) taking one or more electrical and/or optical measurements as the target polynucleotide moves relative to the pore. The present invention also provides an adaptor for characterizing a polynucleotide of interest, wherein the adaptor comprises a modified moiety that binds to a sequencing terminus of the polynucleotide of interest, the modified moiety clogging the nanopore . The method provided by the present invention avoids a large amount of enrichment of the sequenced library at the trans (Trans) end of the nanopore sequencing device, and further improves the variability of the trans (Trans) end modification. |
| priorityDate | 2022-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 398.