abstract |
The invention discloses an electrochemical immunosensor of benzopyrene in seawater and a detection method thereof. The invention firstly uses the drop coating technology to modify the amino-rich carbon nanotube/chitosan nanocomposite film on the surface of the glassy carbon electrode, The electrochemical immunosensor was prepared by the adsorption and immobilization of the antibody with the benzopyrene antibody. Then, the differential pulse voltammetry was used to detect the benzopyrene in seawater by using the specific recognition effect between the benzopyrene and the antibody. The benzopyrene electrochemical immunodetection method provided by the invention is not only simple to operate, the required instruments are portable, can realize on-site detection, and has low cost, and also has the advantages of rapidity, high sensitivity, good specificity, good stability and good reproducibility It has the advantages of high accuracy and high accuracy, and provides a new way for the detection of benzopyrene in seawater environment. |