| abstract |
The invention relates to the technical field of nanomaterials for vaccines, in particular to a sepiolite in-situ growth nanozyme and a preparation method and application thereof. The preparation method comprises mixing ferric chloride, ethylene glycol and sodium acetate to form a suspension, Then, the sepiolite is mixed with the suspension, reacted at a temperature of 180-200° C. to obtain a black precipitate, and then washed and dried to obtain the sepiolite in-situ growth nanozyme. Sepiolite nanozymes achieved in situ growth of iron-based nanozymes on the surface of sepiolite rod-like nanostructures under hydrothermal conditions, which significantly enhanced the peroxidase activity. After Sep@IONzyme is connected to the inactivated influenza virus, the complex forms an appropriate distribution of positive charges, which enhances the adhesion of the antigen to the nasal mucosa. Using it as a nasal vaccine to immunize mice significantly improves the respiratory mucosa-specific sIgA Antibody levels and serum-specific IgG antibody levels provide complete protection against lethal challenge with influenza virus. |