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publicationDate 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114895021-A
titleOfInvention Multi-antibiotic visual detection test strip based on strand displacement-surface proximity hybridization and preparation method and application thereof
abstract The invention belongs to the field of antibiotic detection, and relates to a visual detection test strip, in particular to a multi-antibiotic visual detection test strip based on strand displacement-surface proximity hybridization, and a preparation method and application thereof. The detection test paper adopts a circular design, and the spotting hole is at the center of the circle, which can be divided into multiple detection areas according to the types of antibiotics to be detected. The strand displacement probe and the aptamer are pre-annealed and hybridized to form a complex, which is added to the sample extract together with the signal probe labeled with the optical nanomaterial carbon dots, mixed well, and added dropwise to the central sample area of the detection test paper. In the presence of the target, the strand-displacement probe is competed with the signal probe, and the extraction solution continues to diffuse out along the test paper and migrate to the detection line. In this detection scheme, the brightness of the detection band is proportional to the concentration of the target, which is a signal-on detection mode. The method is economical, rapid, has good specificity and high accuracy, and is expected to be used for the rapid detection of oxytetracycline and chloramphenicol in the market demand.
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