| abstract |
The invention discloses a liquid chromatography detection method for six kinds of alkaloids in tobacco and tobacco products. Wetting, adding triethylamine/ethyl acetate solution, ultrasonicating for a period of time, taking the upper layer liquid, and filtering to obtain filtrate; (2) detection: adopt high performance liquid chromatography to detect, namely obtain six kinds of alkaloids in tobacco and tobacco products content. The detection method of the invention can fully extract the alkaloids in tobacco and tobacco products at one time, the chromatographic peaks of the six alkaloids have good separation effect, and the chromatographic analysis time is shortened to 10 minutes. |