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filingDate 2022-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ac96f98faa802a4b268904bb5e8f32f
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publicationDate 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114894724-A
titleOfInvention A kind of detection method of cloverleaf medicinal substance
abstract The present invention provides a method for detecting medicinal substances of cloverleaf, aiming to solve the problem that in the prior art, medicinal substances with different polarities in cloverleaf need to be extracted and detected separately, and the same extraction conditions cannot be used to simultaneously extract and then problem with detection. The detection method includes: dissolving 1 part by mass of cloverleaf powder in 20-35 parts by volume of ethanol with a volume fraction of 50%; ultrasonic extraction under the conditions of ultrasonic power of 250-400W and temperature of 40-70°C Medicinal substance for 25-40 minutes; the medicinal substance after ultrasonic extraction is kept at 3-5°C for 22-26 hours; then the absorbance is measured with a full-wavelength microplate reader; according to the measured absorbance, the standard curve method is used The contents of total sugar, total ketone, total saponin, total protein and total phenol were determined; wherein, 1 part by mass: 1 part by volume=1 g: 1 ml. The invention innovatively uses 50% ethanol solvent to ultrasonically extract medicinal substances of different polar substances, completes the determination of the total content of various substances, and improves the utilization rate of raw materials.
priorityDate 2022-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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