http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114891728-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-1353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-70 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0645 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0654 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0786 |
| filingDate | 2022-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114891728-B |
| titleOfInvention | Polyelectrolyte membranes, macrophage exosomes and their application in promoting differentiation of BMSCs |
| abstract | The invention discloses a polyelectrolyte membrane, macrophage exosomes and their application in promoting the differentiation of bone marrow mesenchymal stem cells into osteoblasts. The preparation method of macrophage exosomes of the present invention comprises the following steps: take mononuclear macrophages, add phorbol ester to culture for 2 to 3 days, THP‑1 cells differentiate into M0 macrophages, and inoculate M0 macrophages in In a culture vessel coated with a polyelectrolyte membrane, centrifuge after culturing to collect the culture supernatant containing macrophage exosomes; extract the macrophage exosomes from the culture supernatant. The macrophage exosomes of the present invention can promote the differentiation of bone marrow mesenchymal stem cells into osteoblasts. The polyelectrolyte membrane material of the present invention can affect the osteogenic function of stem cells by regulating the behavior of macrophages, and finally obtain a polyelectrolyte membrane coating material that can effectively regulate the activity of macrophages and promote the osteogenesis of stem cells, truly realizing Promote the effect of tissue regeneration and repair in vivo. |
| priorityDate | 2022-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.