| abstract |
The present invention relates to an avian intestinal epithelial cell and its preparation method and application. An avian intestinal epithelial cell is derived from avian embryo small intestinal segment cells, and the genome contains the large T antigen gene of SV40. The researchers of the present invention found that the integration of the large T antigen gene of SV40 into the genome of the small intestinal segment of avian embryos can enable long-term culture of avian intestinal epithelial cells in vitro, continuous culture for more than 6 months, passage for more than 30 generations, and gene expression It is stable, presents the specific characteristics of intestinal epithelial cells, expresses the marker molecules of intestinal epithelial cells, and is suitable for the related research of avian intestinal health and the development of biological products. |