| abstract |
The invention relates to the technical field of biological detection, in particular to a fluorescent microsphere immunochromatographic detection kit for Clostridium perfringens alpha toxin antibody and a preparation method thereof. The kit includes at least a test strip, and the test strip is sequentially provided with a sample pad, a binding pad, a nitrocellulose membrane and a water-absorbing pad from the sample-adding end; The nitrocellulose membrane is equipped with a quality control line on the side near the water-absorbing pad, and the quality control line is sprayed with the monoclonal antibody of the truncated protein of the murine Clostridium perfringens α toxin, and on the side near the sample addition end. There is a detection line, the detection line is sprayed with truncated and expressed C. perfringens alpha toxin protein, and the amino acid sequence of the truncated and expressed C. perfringens alpha toxin protein is shown in SEQ ID NO.4. The invention realizes the detection of the alpha toxin antibody in the sera of various animals, and has wide practicability. |