| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ab0fd83c93e50edc55ad0127c4360199 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-94 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate |
2022-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c964bebe5d249f5e7977186f8f859c00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d72da73ea989f3df36b7691044f52c63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68b777903025ded5627295d11579a4ee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8047cec725cd04efbd7373b408d94ef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2280f1545579e5bc669e74bd9bd3e24a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2425a05aa807eeede2281ac55a99b8a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05cb63954a9d943a1f4e028d893f0222
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a867954ee4803cc27be633dfd8f7a97a |
| publicationDate |
2022-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114875177-A |
| titleOfInvention |
A method for detecting tomato yellow leaf curl virus based on Northern hybridization |
| abstract |
The invention relates to the technical field of plant virus detection, in particular to a method for detecting tomato yellow leaf curl virus based on Northern hybridization. The following steps are included: Step 1. Design primers C4-F and C4-R according to the genome sequence of tomato yellow leaf curl virus, and prepare a tomato yellow leaf curl virus-specific DNA probe with digoxigenin label by PCR method; step 2. Tobacco total RNA extraction and separation of total RNA on agarose gel 3 days after infection with tomato yellow leaf curl virus; step 3. The total RNA isolated in step 2 was transferred to a positively charged nylon membrane by siphoning, and after the transfer was completed The total RNA on the membrane was immobilized under a UV cross-linker; step 4. pre-hybridization, and use the probe prepared in step 1 for hybridization; step 5. washing the hybridization membrane and detecting the hybridization signal. The method overcomes the shortcomings of the existing detection technology, and provides a method for sensitive and specific detection of tomato yellow leaf curl virus. |
| priorityDate |
2022-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |