| abstract |
The invention discloses a kit for detecting high-frequency mutation of MMACHC gene using melting curve, which can detect the high-frequency mutation sites of MMACHC gene c.80A>G, c.217C>T, c.567dupT, c.482G>A, The mutation status of c.658_660delAAG and c.609G>A 6 sites is detected, the detection cost is low, the operation is simple, the accuracy is high, and it has good specificity, which can meet the application of clinical screening. |