http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114875112-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d1643812b9a23a51fc9048bc37745958 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate | 2022-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb572b781aef88e472b78ffc814668c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a80c03819d5c1ea571af15f7d4f9112 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d168e73ecf7d5ecd52c3386da761d0c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5dd31d72956511e65fa1b66fc1336aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_950b068bdbcbff415edf0c996192be04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73ab0f7b59455a578745b803df1d5487 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9469aad32e9456025cb2bbcfebba26f |
| publicationDate | 2022-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114875112-A |
| titleOfInvention | A method for rapid identification of psychrophilic extracellular thermostable protease species |
| abstract | The present invention relates to a method for rapidly identifying psychrophilic bacteria extracellular heat-resistant protease species, comprising the following steps: (1) centrifuging psychrophilic bacteria fermentation broth to prepare fermentation broth samples; (2) configuring SDS-PAGE containing protein substrates gel; (3) mix the fermentation broth sample with the protein loading buffer and load the sample; (4) electrophoresis; (5) take out the gel and perform gel elution with pre-cooled eluent; (6) The gel was incubated in incubation buffer; (7) Coomassie brilliant blue staining. Compared with the prior art, the method of the present invention can rapidly characterize the extracellular heat-resistant protease species of psychrophilic bacteria, can distinguish the heat-resistant protease species of different molecular weights well, and can more accurately characterize the heat-resistant protease species than the traditional method. The tedious protease separation and purification steps are omitted, the repeatability is good, the sensitivity is high, and it can be used for the detection of extracellular heat-resistant proteases of different psychrophilic bacteria, and has a wide application space. |
| priorityDate | 2022-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.