| abstract |
The invention discloses a method for inducing and expanding NK cells in vitro by adopting plant extracts. The induction medium is obtained by adding OK-432, IL-2, IL-15, IL-21 and compound traditional Chinese medicine extracts to Cellgro basic medium. ; Add rhGM-CSF, rhIL-4, compound traditional Chinese medicine extract to Cellgro basal medium to obtain proliferation medium; Aseptically collect peripheral blood, add DPBS to the peripheral blood for dilution, then add Ficoll separation liquid to it, centrifuge, Washing to obtain peripheral blood mononuclear cells; diluting the obtained peripheral blood mononuclear cells, then adding in induction medium to resuspend, adding anti-CD137, and culturing for 2-3 hours; then adding proliferation medium and culturing to the 4th day, supplemented with IL ‑2, IL‑15 and IL‑21; cultured to day 7, expanded in flasks; cultured to day 14 to obtain expanded NK cells. |