| abstract |
The invention discloses a bionic system for simulating lung tissue, a manufacturing method thereof, and a method for controlling microfluid by using the bionic system, wherein the bionic system comprises lung epithelial cells, lung fibroblasts and human umbilical cells isolated from human lungs Venous vascular endothelial cells, and perfused with microfluidics. The chambers inside the system can be perfused with gas and fluid including culture medium, respectively, and similar respiration motion can be simulated, even after more than a week after perfusion with the fluid, the three kinds of cells in the system can survive. In addition, the pH and pO 2 in the chamber can be monitored using the pH measuring sensor and the gas partial pressure measuring sensor inside the system, so that the three kinds of cells inside the system are exposed to the external environment under the same conditions as in vivo lungs or drugs, etc. As a result, research in a wide range of fields including lung disease modeling caused by harmful substances, pharmacodynamic testing of therapeutic drugs, etc. can be realized, and further, it can be applied to in vitro disease modeling and customized drug prescription. |