| abstract |
The present invention provides an immunoglobulin G4 detection kit and a preparation method thereof. The kit consists of a first reagent R1 and a second reagent R2: the first reagent R1 includes a zwitterion buffer with a concentration of 25-200 mmol/L, 5～10g/L inorganic salt, 0.5w%～5w% surfactant 1 and 0.05w%～1w% preservative; the second reagent R2 includes 25～200mmol/L zwitterionic buffer, 5～10g/L Inorganic salt, 50～100g/L sucrose, 50～100g/L trehalose, 0.5w%～5w% surfactant 2, 1w%～5w% conjugated with immunoglobulin G4 monoclonal antibody labeled by carrier molecule of latex microspheres, 1w% ~ 5w% blocking agent and 0.05w% ~ 1w% preservative. The kit of the invention has the advantages of high coupling efficiency, high sensitivity, anti-hook effect and the like. |