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filingDate 2022-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114858875-A
titleOfInvention A method for self-enhanced photoelectrochemical detection of kanamycin
abstract An amino-functional metal-organic framework (Cu-MOF-NH 2 ) modified copper sheet (Cu-MOF-NH 2 /CuSs) was designed and synthesized. Compared with that before amino functionalization, the band gap of Cu-MOF-NH 2 is reduced from 3.5 eV to 2.6 eV, and the reduction of the band gap enables Cu-MOF-NH 2 to have a wider light absorption range. A photoelectrochemical biosensor for detecting kanamycin was constructed by modifying the kanamycin aptamer to the amino functional group in Cu‑MOF‑NH 2 /CuSs by Schiff base. The holes existing on the amino functional group of Cu-MOF-NH 2 can oxidize kanamycin, making kanamycin itself a signal-enhancing substance to achieve self-enhancing effect. The photoelectrochemical biosensor has a detection range of 0.5 nM to 650 nM with a detection limit of 0.1 nM. In addition, the biosensor has good stability, reproducibility and selectivity. The detection results of the actual samples were also satisfactory. The recovery rates for the detection of kanamycin content in fish were 95.7%-105.0%, and the relative standard deviations were 3.5%-4.9%.
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