| abstract |
The invention discloses special primers and kits for identifying Pediococcus pentosaceus. The special primers for identifying Pediococcus pentosaceus disclosed in the present invention are composed of primers named F3, B3, FIP, BIP, LF and LB respectively, and F3, B3, FIP, BIP, LF and LB are the sequences in the sequence table respectively. Single-stranded DNA shown in 1-6. The special primer for identifying Pediococcus pentosaceus can detect Pediococcus pentosaceus, and has the advantages of high sensitivity, strong specificity, simple operation, quickness and convenience, etc. The loop-mediated isothermal amplification method is used to amplify the specificity of Pediococcus pentosaceus. The sex gene fragment fills the blank of the LAMP detection method of Pediococcus pentosaceus in beer, and the invention is suitable for basic application in winery and rapid on-site detection. |