| abstract |
A fluorescent PCR detection kit for β-lactamase resistance gene bla Z and application thereof belong to the field of biotechnology. On the one hand, the present invention provides a fluorescent PCR detection kit for the β-lactamase resistance gene bla Z, and on the other hand, provides an application and an application method of the kit. The invention has the following beneficial effects: a pair of primers and a probe are specifically designed for the β-lactamase resistance gene bla Z, with high specificity and accurate detection results; the kit of the invention has reasonable components and proportions, The operation is simple, the use is convenient, and the cross-contamination is reduced to the greatest extent; the invention simplifies the detection process, reduces the time by two days compared with the traditional culture analysis method, and greatly shortens the detection period. |