| abstract |
The invention relates to a rapid micro-inoculation method for pathogenic bacteria of corn stem diseases, belonging to the technical field of microorganisms. The method is as follows: using a sterilized blade to cut a small incision at the leaf sheath and/or stalk in the middle of the corn plant, inserting the pathogenic bacteria into the wound with a filter paper sheet, and moisturizing the corn plant inoculated with the bacteria. The inoculation method can meet the rapid detection of pathogenicity of various diseases and pathogenic bacteria of corn stem, and can be used for the screening of disease resistance of corn varieties at seedling stage, the screening of pathogen control agents, and for the resistance of stalk fiber tissue in corn seedling stage and adult stage. Stem rot ability detection is of great significance for carrying out research on pathogenicity, diversity, variety resistance, and disease control. |