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filingDate 2021-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa7e1f5c0b80d9eed650117994330e83
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publicationDate 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114854592-A
titleOfInvention A kind of deodorizing lactic acid bacteria screening method
abstract The invention discloses a screening method for deodorizing lactic acid bacteria. The raw materials include 505 grams of fresh pig manure, 100 milliliters of sterile water, 20 milliliters of boric acid solution, 20 milliliters of zinc-ammonium complex salt solution, 50 milliliters of MRS culture medium, and MRS liquid Culture medium, conical flask, coating on MRS solid plate, wide-mouthed flask, shaking flask, shaker and beaker, take 5 grams of fresh pig manure in a triangular flask with sterile water, put it in a shaker, and put it into a shaker at temperature. After being treated for 2 hours at 30° C. and rotating speed of 200 rpm, it was taken out, and left to stand for 1 hour. Take 5 ml of the supernatant into a conical flask containing MRS medium, put it into a shaker, and treat it in a shaker with a temperature of 30 ° C and a rotating speed of 120 rpm for 48 hours, and the sample will be sampled by 10-fold dilution method. , draw 0.1 ml from the dilution of 10-3, 10-5, 10-7, 10-9 and apply it on the MRS solid plate, and cultivate at 30 ℃ constant temperature. It has good deodorizing effect, accurate screening results and simple screening process. advantage.
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