| abstract |
The invention belongs to the technical field of biological materials, and discloses a suspension medium for suspending 3D bioprinting and a preparation method and application thereof. The suspension medium of the present invention includes but is not limited to the following components in mass percentage: 0-10% of gellan gum, 0-10% of mercapto-gellan gum, and the remainder of solvent; and when the contents of gellan gum and mercapto-gellan gum are different zero. The suspension medium of the invention has good thixotropy and self-healing ability, so that the nozzle of the 3D bioprinter can move freely in the suspension medium, and at the same time, the integrity of the 3D bioprinting structure can be maintained; The problem that natural polymer materials such as fibrin cannot achieve self-supporting printing, and the printing accuracy can reach 200 microns and the fidelity can reach 99%. It is expected to be used for the printing of tissue and organ structures loaded with cells and active substances in vitro. industrialization prospects. |