| abstract |
A method for analyzing disaccharide compounds, which relates to the field of medical detection, and the purpose of the present invention is to provide a method for analyzing related substances in lactulose solution with high sensitivity and good stability. The chromatographic conditions are as follows: the chromatographic column adopts an aminopropyl chromatographic column; sodium dihydrogen phosphate solution-acetonitrile (10:90～30:70), isocratic elution; the column temperature is 30 ℃～50 ℃; the flow rate is 0.5mL/ min～30mL/min; injection volume 20μl; the detector is a refractive index detector (RID), the detector temperature is 30℃～50℃; the sample concentration is 1mg/mL～100mg/mL. The invention has the advantages of high detection sensitivity, good reproducibility and high stability, and is suitable for the detection of lactulose related substances. |