| abstract |
The invention relates to the technical field of detection, in particular to a non-disease diagnosis purpose amanita venom peptide detection method. The detection method includes the operation of detecting the amanita peptide in the sample to be tested, and the sample to be tested is prepared by subjecting the sample to be tested to protein degradation. The sample to be tested used in the detection method is prepared by subjecting the sample to be detected by protein degradation treatment, wherein the protein in the protein-bound amanita toxin can be degraded by the protein degradation treatment, thereby releasing more free amanita toxin. peptides. Therefore, the free state amanita toxin contained in the sample to be tested used in the present invention is composed of the original free state amanita toxin and the free state amanita toxin released by the protein-bound state amanita toxin, that is, the present invention The detection method can simultaneously detect the free and protein-bound amanita peptides contained in the sample to be tested, which can significantly extend the detection window period of amanita peptides and effectively improve the detection rate of amanita toxins. |