http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114836516-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a0598d42aa7727385b85c1cc69c811a1 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P60-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-946 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-928 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-942 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-54 |
| filingDate | 2022-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32d1dafb3d6bb9289eb443909c3b3c43 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_623241ae80ff50fe20f473c967e7a4e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59240d1892e24075b8666384ee3c3a22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3ddec54cd94b49f7d1ea53a5d3cacf6 |
| publicationDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114836516-A |
| titleOfInvention | Method for rapid detection of non-starch polysaccharide enzyme titer for feed |
| abstract | The present application relates to the field of quality evaluation of feed compound enzymes, and specifically discloses a method for rapidly detecting the titer of non-starch polysaccharide enzymes used in feed, comprising the following steps: S1. Drawing a standard curve: first, draw a standard for glucose absorbance value and glucose concentration Curve; S2. De-starchization of feed: mix the feed with a weight ratio of 1:(2-5) and water, then add α-amylase to react for 3 hours, then add saccharification enzyme to react for 4 hours, and after standing for 2-3 hours, centrifuge Take the sediment to get the de-starchized feed; S3. In vitro digestion stage: perform in vitro enzymatic digestion on the de-amylized feed and the enzyme solution to be tested to obtain the sample to be tested, and measure the absorbance of the sample to be tested and the blank sample; S4. Determination Reducing sugar: Calculate the reducing sugar content according to the standard curve in S1 from the absorbance measured in S3, and calculate the reducing sugar increment according to the formula, which can improve the accuracy of evaluating the effect of non-starch polysaccharide enzymes in the feed on the feed. advantage. |
| priorityDate | 2022-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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