| abstract |
The present invention relates to a method for preparing polypeptides by using eggshell membranes, the prepared polypeptides and applications, including the following steps S1, steam explosion treatment: placing the eggshell membranes in a steam explosion machine reactor, under a pressure of 1.0-1.4MPa Pressurize for 1 to 5 minutes, then release the pressure instantaneously, and collect the steam explosion samples; S2, protease enzymolysis treatment: add the eggshell membrane after steam explosion treatment to a pH of 1: (10-30) with a solid-liquid ratio of 1: 7-10% of sodium hydroxide aqueous solution, then add alkaline protease according to the amount of enzyme added 2000-14000U/g, enzymolysis at 35-65 ℃ for 2-14 hours, inactivate the enzyme at 90-100 ℃ for 5-15 minutes, The enzymatic hydrolyzate was filtered, the filtrate was centrifuged, and the supernatant was freeze-dried to obtain the polypeptide. Based on the method of the present invention, the preparation efficiency is high and the prepared polypeptide has high antioxidant activity. |