| abstract |
The invention discloses a preparation method of human mesenchymal stem cell secretion factor liposome freeze-dried powder, which comprises the following steps: firstly dissolving 35-75 parts by weight of liposome membrane components in an organic solvent, and rotary evaporation to remove all organic solvents A liposome film is obtained, and then a buffer solution containing 10-40 parts by weight of a freeze-drying protective agent is added to the film for hydration, and ultrasonic granulation is performed to obtain a blank liposome suspension. Then 1-10 parts by weight of human mesenchymal stem cells secreted factors and 1-10 parts by weight of antioxidants are added to the blank liposome suspension for encapsulation reaction, the product is subjected to membrane granulation, and then freeze-thawed and frozen in turn. It is obtained after drying. The liposome freeze-dried powder of the invention has a long shelf life and wide application range. |