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filingDate 2022-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7cfa2c3043f8593c988621617386681
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publicationDate 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114814205-A
titleOfInvention A quintuplex fluorescent immunoquantitative test strip for simultaneous detection of multiple mycotoxins and heavy metal Cd in grains
abstract The invention discloses a quintuple fluorescent immunoquantitative test strip for simultaneous detection of multiple mycotoxins and heavy metal Cd in grains, belonging to the technical field of rapid detection of immunoassays. In the present invention, the method for preparing the five-unit fluorescence immunoassay quantitative test strip includes the following steps: respectively applying 0.01-1.0 mg/mL AFB1, FB1, OTA, and ZEN complete antigen solutions on the NC membrane as detection lines T1-T4; ‑1.0mg/mL Cd complete antigen solution was applied to the NC membrane as the detection line T2, 0.05‑1mg/mL goat anti-mouse secondary antibody solution was applied to the nitrocellulose membrane as the quality control line C, and dried; , The nitrocellulose membrane containing the detection line and the quality control line, and the absorbent paper are pasted on the PVC bottom plate in turn to form a test strip. The five-unit fluorescent immunoquantitative test strip of the invention can simultaneously detect various mycotoxins and heavy metal Cd in grains on one test strip, and has the advantages of high sensitivity, low cost, simple operation, rapid quantitative detection and good stability. It has the advantages of good commercial application prospects.
priorityDate 2022-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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