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filingDate 2022-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c74f9c089b675aabffc161972eb2efaf
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publicationDate 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114813288-A
titleOfInvention A method for rapid staining and localization of reactive oxygen species in plant roots
abstract The present invention provides a method for rapid dyeing and positioning of plant root reactive oxygen species. Vacuum staining; (2) discard the staining solution, add fixative, put it into a vacuum container, and perform low-temperature vacuum fixation; (3) discard the fixative, add glycerin aqueous solution, put it into a vacuum container, and perform low-temperature vacuum filling and shaping; (4) ) Pre-cool the cold room and the slicing cutter, embed the plant roots discarded from the aqueous glycerol solution with an OTC embedding medium, adjust the temperature of the fixing table, and perform slicing; (5) The plant roots are returned to temperature and then sealed. The method of the invention saves a lot of production time, realizes rapid slicing; reduces the influence of temperature on the metabolism of reactive oxygen species, greatly improves the accuracy of slicing; can ensure that the tissue will not shrink, maintain the original living form, and ensure the slicing authentic and reliable.
priorityDate 2022-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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