| abstract |
The invention provides a primer and a probe for the detection of pigeon rotavirus type A fluorescence quantitative PCR, and a kit thereof. The primer sequences are as follows: primer PiRVA-TF: 5'-CCGAACTGCTCCTGTATGAAT-3', primer PiRVA-TR: 5 '-CATTGCCCATTGCTATCCATTT-3'; the probe is: probe PiRVA-probe: 5'-AGTCTGACATCCATTTCATTGCACAACC-3', and its 5'-end is labeled with a fluorescent reporter group FAM, at the middle and 3'-end of the probe The quenching group DBQ1 is marked respectively. The method established by the present invention has high sensitivity, good stability, strong specificity and good repeatability, and can be used for the specific detection of pigeon rotavirus type A, which is the basis for subsequent scientific research of pigeon rotavirus type A. Pathogenic mechanisms and the development of molecular epidemiology laid the foundation. |