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filingDate 2022-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b61c1a22b02b635cea24f5d00773a32
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publicationDate 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114807439-A
titleOfInvention Clinical application of multiplex PCR technique for detection of drug resistance genes of multiple pathogens at the same time
abstract The invention relates to the technical field of pathogen detection, in particular to the clinical application of multiplex PCR technology to detect multiple pathogen resistance genes at the same time; the invention extracts nucleic acid by an automatic nucleic acid extraction instrument by breaking, centrifuging, and lysing the sample, and the extracted Nucleic acids are dissolved in DEPC water, drug resistance genes are added, and Takara reverse transcriptase is used for reverse transcription. PCR amplification, sequencing and analysis of the reverse transcription products can effectively improve the detection performance of various pathogens. Compared with the traditional direct immunofluorescence method, it is more superior and can be applied in the clinic of drug resistance genes of various pathogens.
priorityDate 2022-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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