| abstract |
The invention discloses a primer mixture, a kit and a detection method for detecting N. americana. The primer mixture comprises: primer F3.3, whose nucleotide sequence is shown in SEQ ID NO: 1; primer B3.3, whose nucleotide sequence is shown in SEQ ID NO: 1; The nucleotide sequence is shown in SEQ ID NO: 2; the primer FIP3, its nucleotide sequence is shown in SEQ ID NO: 3; the primer BIP3, its nucleotide sequence is shown in SEQ ID NO: 4; the primer LF3, Its nucleotide sequence is shown in SEQ ID NO: 5; the nucleotide sequence of primer LB3 is shown in SEQ ID NO: 6, and the present invention also provides a kind of described primer mixture in the kit for detecting N. americana The application of American hookworm detection system, detection method. The advantages of the present invention include: short amplification time, simple operation, low cost, high sensitivity and good specificity. |