abstract |
The present invention relates to a chimeric antigen receptor comprising a costimulatory receptor, and the structure of the chimeric antigen receptor is scFv(X)-(Y)CD3zeta-2A-(Z); wherein, X includes a tumor-targeting antibody Or a ligand or receptor that can specifically bind to a tumor; Y is the intracellular region of a costimulatory receptor, Z is a costimulatory receptor, and the costimulatory receptor is selected from ICOS, CD28, CD27, HVEM, LIGHT, CD40L , 4‑1BB, OX40, DR3, GITR, CD30, TIM1, SLAM, CD2, CD226. The present invention also provides a CAR-T cell constructed from the above-mentioned chimeric antigen receptor recombinant expression vector, and a preparation method and application thereof. The CAR-T cells of the present invention significantly improve tumor killing ability and expansion ability. |