| abstract |
The invention discloses an in vitro diagnostic reagent for combined detection of pulmonary tuberculosis-lung cancer disease. The combined detection of pulmonary tuberculosis-lung cancer was performed by colloidal gold immunochromatography. The optimal coating concentration of MPT-64 recombinant protein and CFP-10 recombinant protein labeled by the detection line of tuberculosis test strip was 2.5mg/mL, and the mouse anti-human CYFRA21-1 monoclonal antibody and The optimal coating concentration of mouse anti-human NSE monoclonal antibody was 4.0 ng/mL, and the optimal coating concentration of mouse anti-rabbit IgG monoclonal antibody labeled with the quality control line was 1.5 mg/mL. Nitrocellulose membrane (NC membrane) should choose Immunodyne ABC membrane from American PALL Company. The glass fiber membrane should be SB08 glass fiber membrane, and the blocking solution prepared with 10% BSA should be selected. The invention uses a biotin-streptavidin signal amplification system, adopts a one-card two-channel detection mode, and jointly detects pulmonary tuberculosis-lung cancer, which has the advantages of simplicity, sensitivity, rapidity, good stability, etc., and is used for the serological diagnosis of two diseases. Offers new ideas and means. |