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publicationDate 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114791491-A
titleOfInvention A method for rapid detection of Staphylococcus aureus using biofilm interference technology
abstract The invention discloses a method for rapidly detecting Staphylococcus aureus by using biofilm interference technology. The method comprises the following steps: step 1, sensor balance; step 2, sensor activation; step 3, antibody immobilization; step 4, primary sealing; Fifth, secondary sealing; Step six, sample detection and result analysis. The beneficial effects of the invention are as follows: few steps, no labels, real-time monitoring, convenience and quickness, and simple result judgment; the operation is simple and easy to learn; The presence of Staphylococcus aureus can be determined by taking the binding signal; the antibody diluted with acetic acid-sodium acetate buffer solution can be recovered and reused to reduce the detection cost; high-throughput detection can be used with the corresponding biomolecular interaction instrument and supporting The sample plate can realize the simultaneous detection of up to 95 samples; it has good specificity and can exclude the interference of non-target bacteria in the sample.
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