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filingDate 2022-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca51e02a8c747dab4ed5ccd9d58bd702
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publicationDate 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114790491-A
titleOfInvention A DNA probe for detecting nucleic acid of urogenital tract infection pathogen and its application
abstract The invention discloses a DNA probe for detecting nucleic acid of urogenital tract infection pathogens and its application. The urogenital tract infection pathogens include Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium. The DNA probe includes probe H1 and probe H2. DNA The probe is mixed with the target sequence to catalyze the hairpin self-assembly reaction. The 5' end of probe H1 is modified by digoxigenin, and the 5' end of probe H2 is modified by biotin. The invention utilizes DNA probes and target sequences to mix catalyzed hairpin self-assembly reaction combined with immunochromatographic test strips, and can detect Chlamydia trachomatis and Neisseria gonorrhoeae rapidly, with high sensitivity and high specificity under the environment of isothermal enzyme-free amplification. bacteria and Mycoplasma genitalium.
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