| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cbd749eb61f56e76c45e6b8351d8062 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4703 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-548
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-89
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-89
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-548
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate |
2022-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cea6f3aec7aab5d17586005fbebd0111
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_487f33bdbfb9ff4fead7e7fb19514c64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1df0fe93c3e7cf2a0845955be5be71b |
| publicationDate |
2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114774524-A |
| titleOfInvention |
Application of miR-491 and miR-381 in the regulation of post-transcriptional expression of CART in bovine hypothalamus |
| abstract |
The invention discloses the application of miR-491 and miR-381 in the post-transcriptional expression regulation of CART in bovine hypothalamus, including the comparative analysis of the inhibitory effect of miRNAs on CART; the detection of 293T cell gene expression, including the extraction and detection of total cell RNA; Transcription; miRNA reverse transcription; primer design; fluorescence quantitative PCR detection; detection of CART protein expression in 293T cells, including total cell protein extraction; protein concentration determination by BCA method; sample loading; electrophoresis; membrane transfer; blocking; primary and secondary antibodies Incubation; development; regulation of CART expression in mice by miR-491 and miR-381, including detection of gene expression in mouse hypothalamus by qRT-PCR; detection of CART protein expression in mouse hypothalamus by Western blot; detection of CART in mouse serum by Elisa concentration. |
| priorityDate |
2022-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |