http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114774458-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db9efde660b78f98b102046e09d07ab7 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
| filingDate | 2022-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c22d23a38339928becc485e7af6af6ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc454b62bcfa797aad2d6b24033f5412 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfcf14635bbf0b9d10225354ea80c4f2 |
| publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114774458-A |
| titleOfInvention | A genetic method for in situ expression of fluorescent fusion protein of filamentous fungi and its application |
| abstract | The invention discloses a gene method for in situ expression of fluorescent fusion protein of filamentous fungi and its application. It belongs to the field of molecular biology and biotechnology; the specific steps are: 1. construction of a fluorescent protein carrier; 2. construction of a fluorescent fusion protein in situ expression plasmid; 3. fungal transformation to construct a recombinant strain; Fusion protein confocal imaging and quantitative analysis of fluorescent fusion proteins). In the present invention, a fluorescent protein gene is in situ connected to the 3' end of the fungal target gene by means of homologous recombination gene manipulation mediated by Agrobacterium, so that the target gene and the fluorescent protein gene are co-expressed in the form of fusion protein (target protein-fluorescent protein). The subcellular localization of the target protein is analyzed by fluorescence observation and its expression is quantified; the fungal fluorescent fusion protein in situ gene expression technology disclosed in the present invention reduces the uncertainty of random insertion and improves the accuracy of the experimental results. |
| priorityDate | 2022-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.