| abstract |
The invention relates to an application and method of a tobacco osmotic protein-related gene, and belongs to the technical field of plant genetic engineering. The present invention constructs a CRISPR/Cas9 editing vector for knocking out the osmotic protein gene through the CRISPR/Cas9-mediated gene editing technology, and obtains a safflower Dajinyuan plant with the osmotic protein gene edited after genetic transformation. The invention utilizes CRISPR/Cas9-mediated gene editing technology to knock out the osmolyte gene to obtain gene editing plants that play an important role in influencing proline content and stress resistance. Stress resistance research provides genetic material and theoretical basis. |